Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAP

Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAP

Blog Article

Modern microscopy methods are powerful tools Goalie - Cages for studying live cell signaling and biochemical reactions, enabling us to observe when and where these reactions take place from the level of a cell down to single molecules.With microscopy, each cell or molecule can be observed both before and after a given perturbation, facilitating better inference of cause and effect than is possible with destructive modes of signaling quantitation.As many inputs to cell signaling and biochemical systems originate as protein-protein interactions near the cell membrane, an outstanding challenge lies in Acrylic Family Puzzle controlling the timing, location and the magnitude of protein-protein interactions in these unique environments.

Here, we detail our procedure for manipulating such spatial and temporal protein-protein interactions in a closed microscopy system using a LOVTRAP-based light-responsive protein-protein interaction system on a supported lipid bilayer.The system responds in seconds and can pattern details down to the one micron level.We used this technique to unlock fundamental aspects of T cell signaling, and this approach is generalizable to many other cell signaling and biochemical contexts.